Journal: Orphanet Journal of Rare Diseases
Article Title: In vitro characterization of osteoblasts from craniofacial fibrous dysplasia of bone and their impact on bone homeostasis
doi: 10.1186/s13023-026-04262-0
Figure Lengend Snippet: FD osteoblasts exhibit defective phenotype. ALP quantification ( A ) and staining ( B ). Calcium concentration ( C ) and Alizarin Red staining ( D ). Scale bar: 75 μm ( B , D ); COL1A1 ( E ) and BGLAP ( F ) gene expression measurement by RT-qPCR. ( G ) Representative photographs of FD and non-FD cell cultures exhibit different type-I collagen and osteocalcin expression at D21 of culture. Type-I collagen (a, b) and Osteocalcin (c, d) immunostainings. Colorized overlayed images (e, f): ColIa1 (AlexaFluor488)/OC (AlexaFluor568)/nucleus (DAPI). Grey values represent non-FD osteoblasts and black values represent FD osteoblasts. Images were acquired with a Zeiss Axiovert 200 M inverted microscope. Scale bar: 20 μm (G: a,b, c,d, e,f). FD, n = 7; non-FD, n = 8. Δ p < 0.05 FD versus non-FD; # p < 0.05 versus D7; £ p < 0.05 versus D14
Article Snippet: Cells were permeabilized with 3% BSA (Sigma Aldrich, Saint-Quentin Fallavier, France) plus 0.1% Triton X100 (Acros Organics, Thermo Fisher Scientific, Illkirch, France) for 45 min then incubated overnight at 4 °C with rabbit anti-human type-I collagen monoclonal antibody (clone H-197, Santa Cruz Biotechnology, Heidelberg, Germany) and mouse anti-human osteocalcin monoclonal antibody (clone 190125, R&D systems, Lille, France).
Techniques: Staining, Concentration Assay, Gene Expression, Quantitative RT-PCR, Expressing, Inverted Microscopy