bio rad 0400 0041  (Bio-Rad)

Journal: Polymers

Article Title: Simple Electrospinning Method for Biocompatible Polycaprolactone β-Carotene Scaffolds: Advantages and Limitations

doi: 10.3390/polym16101371

Figure Lengend Snippet: Biological testing results with KUSA-A1 and E. coli : ( a ) KUSA-A1 cell optical density, ( b ) area coverage of the osteocalcin staining with respect to the investigated area of the sample, ( c ) E. coli optical density. Results marked with “*” have a p -value < 0.05 when compared with all others.

Article Snippet: Osteogenic differentiation was assessed after 10 days in the osteogenic medium by staining cultured KUSA-A1 cells with the green osteocalcin stain (Osteocalcin Mouse anti-Human, PE, Clone, Fisher Scientific, Hampton, NH, USA) following the manufacturer’s instructions.

Techniques: Staining

Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Article Title: Additional Insights Into the Role of Osteocalcin in Osteoblast Differentiation and in the Early Steps of Developing Alveolar Process of Rat Molars.

doi: 10.1369/00221554231211630

Figure Lengend Snippet: Figure 3. (A–F) Light micrographs showing portions of non-decalcified heads of 16-, 18- and 20-day-old rat fetuses. The sections were subjected to the von Kossa histochemical method followed by immunohistochemistry for the detection of Osx (A–C) and OCN

Article Snippet: Afterward, sections were incubated in a humidified chamber at 4°C overnight in each of the following primary antibodies: mouse monoclonal anti-OCN antibody (MAB1419, R&D Systems, diluted 1:600) and rabbit polyclonal anti-ALP antibody (sc30203; Santa Cruz Biotechnology, Dallas, TX, diluted 1:1000).

Techniques: Immunohistochemistry

Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Article Title: Additional Insights Into the Role of Osteocalcin in Osteoblast Differentiation and in the Early Steps of Developing Alveolar Process of Rat Molars.

doi: 10.1369/00221554231211630

Figure Lengend Snippet: Figure 6. (A and B) Number of ALP-immunolabelled (A) and OCN-immunolabelled (B) cells (mean ± SD) in cells of dental follicle/ developing periodontal ligament of rat fetuses at 16, 18, and 20 days (E16, E18, and E20) and 5-, 10-, and 15-day-old rats (P05, P10, and P15). Superscript asterisks indicate significant differences between the groups. ANOVA/Tukey’s test. Abbreviations: ALP, alkaline phosphatase; OCN, osteocalcin. *p<0.05, **p<0.01, ****p<0.0001.

Article Snippet: Afterward, sections were incubated in a humidified chamber at 4°C overnight in each of the following primary antibodies: mouse monoclonal anti-OCN antibody (MAB1419, R&D Systems, diluted 1:600) and rabbit polyclonal anti-ALP antibody (sc30203; Santa Cruz Biotechnology, Dallas, TX, diluted 1:1000).

Techniques:

Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Article Title: Additional Insights Into the Role of Osteocalcin in Osteoblast Differentiation and in the Early Steps of Developing Alveolar Process of Rat Molars.

doi: 10.1369/00221554231211630

Figure Lengend Snippet: Figure 8. (A–F) Electron micrographs showing portions of bone matrix (BM) in early stages of formation from rat fetuses at 16 (A–D and F) and 20 (E) days. Sections of LR White-embedded specimens subjected to the immunolocalization of osteocalcin (OCN).

Article Snippet: Afterward, sections were incubated in a humidified chamber at 4°C overnight in each of the following primary antibodies: mouse monoclonal anti-OCN antibody (MAB1419, R&D Systems, diluted 1:600) and rabbit polyclonal anti-ALP antibody (sc30203; Santa Cruz Biotechnology, Dallas, TX, diluted 1:1000).

Techniques:

Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Article Title: Additional Insights Into the Role of Osteocalcin in Osteoblast Differentiation and in the Early Steps of Developing Alveolar Process of Rat Molars.

doi: 10.1369/00221554231211630

Figure Lengend Snippet: Figure 9. (A–D) Electron micrographs of portions of developing bone matrix (BM) of 5- (A), 10- (B and C), and 15-day-old (D) rats. Sections of Araldite-embedded specimens (A and D) and sections of LR White-embedded specimens (B and C). (A) Polarized osteo- blasts (Ob) containing several profiles of rough endoplasmic reticulum (rER) are adjacent to mineralizing BM. The inset, outlined area in (A), shows a matrix vesicle (MV) in the BM. (B and C) Sections subjected to the immunolocalization of OCN. An Ob adjacent to the non-mineralized BM in formation (asterisks). Higher magnification of non-mineralized matrix (C), gold particles (arrows) indicating OCN immunoreactivity are observed mainly in close association with collagen fibrils (Cf). Few gold particles (white arrows) are seen in the BM in the advanced stage of mineralization. In (D), Ob are juxtaposed to the BM in the advanced stage of development of the alveolar process. Scale bars: 2.5 µm (A and D), 1 µm (B), 0.1 µm (C), 0.4 µm [inset of (A)].

Article Snippet: Afterward, sections were incubated in a humidified chamber at 4°C overnight in each of the following primary antibodies: mouse monoclonal anti-OCN antibody (MAB1419, R&D Systems, diluted 1:600) and rabbit polyclonal anti-ALP antibody (sc30203; Santa Cruz Biotechnology, Dallas, TX, diluted 1:1000).

Techniques:

Journal: Materials

Article Title: Evaluation of Hard and Soft Tissue Responses to Four Different Generation Bioresorbable Materials-Poly-l-Lactic Acid (PLLA), Poly-l-Lactic Acid/Polyglycolic Acid (PLLA/PGA), Uncalcined/Unsintered Hydroxyapatite/Poly-l-Lactic Acid (u-HA/PLLA) and Uncalcined/Unsintered Hydroxyapatite/Poly-l-Lactic Acid/Polyglycolic Acid (u-HA/PLLA/PGA) in Maxillofacial Surgery: An In-Vivo Animal Study

doi: 10.3390/ma16237379

Figure Lengend Snippet: OCN intensity measurement using DAB color deconvolution and results from ROI marking in ImageJ. DAB, diaminobenzidine; OCN, osteocalcin; ROI, region of interest.

Article Snippet: For primary staining, the slides were treated with an anti-CD68 rabbit polyclonal antibody (Abcam: ab125212; dilution 1:1000), mouse anti-human osteocalcin monoclonal antibody (BIO-RAD: 0400-0041; dilution 1:3000), and anti- runt-related transcription factor x type 2 (Runx2) rabbit polyclonal antibody (Abcam: ab23981; dilution 1:300) at room temperature for 50 min.

Techniques:

Journal: Materials

Article Title: Evaluation of Hard and Soft Tissue Responses to Four Different Generation Bioresorbable Materials-Poly-l-Lactic Acid (PLLA), Poly-l-Lactic Acid/Polyglycolic Acid (PLLA/PGA), Uncalcined/Unsintered Hydroxyapatite/Poly-l-Lactic Acid (u-HA/PLLA) and Uncalcined/Unsintered Hydroxyapatite/Poly-l-Lactic Acid/Polyglycolic Acid (u-HA/PLLA/PGA) in Maxillofacial Surgery: An In-Vivo Animal Study

doi: 10.3390/ma16237379

Figure Lengend Snippet: OCN expression surrounding biomaterial sheets at weeks 2 and 10. OCN intensity was marked by week 10 in the u-HA/PLLA and u-HA/PLLA/PGA groups (×1.25 magnification, Scalebar = 1 mm). PLLA, poly-L-lactic acid; PGA, polyglycolic acid; u-HA, uncalcined/unsintered hydroxyapatite; OCN, osteocalcin.

Article Snippet: For primary staining, the slides were treated with an anti-CD68 rabbit polyclonal antibody (Abcam: ab125212; dilution 1:1000), mouse anti-human osteocalcin monoclonal antibody (BIO-RAD: 0400-0041; dilution 1:3000), and anti- runt-related transcription factor x type 2 (Runx2) rabbit polyclonal antibody (Abcam: ab23981; dilution 1:300) at room temperature for 50 min.

Techniques: Expressing

Journal: Materials

Article Title: Evaluation of Hard and Soft Tissue Responses to Four Different Generation Bioresorbable Materials-Poly-l-Lactic Acid (PLLA), Poly-l-Lactic Acid/Polyglycolic Acid (PLLA/PGA), Uncalcined/Unsintered Hydroxyapatite/Poly-l-Lactic Acid (u-HA/PLLA) and Uncalcined/Unsintered Hydroxyapatite/Poly-l-Lactic Acid/Polyglycolic Acid (u-HA/PLLA/PGA) in Maxillofacial Surgery: An In-Vivo Animal Study

doi: 10.3390/ma16237379

Figure Lengend Snippet: ( A ) Graph showing OCN accumulation outside sheet. * Indicates statistical significance. OCN, osteocalcin. ( B ) OCN accumulation adjacent to host bone. * Indicates statistical significance. OCN, osteocalcin.

Article Snippet: For primary staining, the slides were treated with an anti-CD68 rabbit polyclonal antibody (Abcam: ab125212; dilution 1:1000), mouse anti-human osteocalcin monoclonal antibody (BIO-RAD: 0400-0041; dilution 1:3000), and anti- runt-related transcription factor x type 2 (Runx2) rabbit polyclonal antibody (Abcam: ab23981; dilution 1:300) at room temperature for 50 min.

Techniques: